| 出品公司: | Promega |
|---|---|
| 载体名称: | pNL1.3[secNluc] |
| 质粒类型: | 荧光素酶表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | --- |
| 启动子: | -- |
| 载体大小: | 3194 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 无启动子载体，用于通过分泌的NanoLuc®荧光素酶检测启动子和增强子序列的活性。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pNL1.3[secNluc]载体图谱](/uploads/载体图谱/pNL1.3[secNluc]载体图谱.png)
载体简介
载体序列
LOCUS pNL1.3[secNluc] 3194 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Promoterless vector for measuring the activity of promoter and
enhancer sequences with a secreted NanoLuc(R) luciferase assay.
ACCESSION JQ437372
VERSION .
KEYWORDS pNL1.3[secNluc]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3194)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Thursday, February 20, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3194
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
CDS 100..186
/codon_start=1
/product="secretion signal from interleukin 6"
/note="IL-6 signal sequence"
/translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAP"
CDS 187..699
/codon_start=1
/product="NanoLuc(R) luciferase"
/note="Nluc"
/note="human codon-optimized"
/translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
LCERILA"
polyA_signal 748..869
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1288..1876)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2076..2936)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 3041..3089
/note="synthetic polyadenylation signal"
misc_feature 3103..3194
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tgaactcctt ctccacaagc
121 gccttcggtc cagttgcctt ctccctgggc ctgctcctgg tgttgcctgc tgccttccct
181 gccccagtct tcacactcga agatttcgtt ggggactggc gacagacagc cggctacaac
241 ctggaccaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc
301 gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat
361 gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt
421 aaggtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg
481 gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc
541 gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt
601 atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga
661 gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaag gccgcgactc tagagtcggg
721 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
781 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
841 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
901 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
961 aaatcgataa ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
1021 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
1081 ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc
1141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
1201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
1261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
1321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
1381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
1441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
1501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
1561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
1621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
1681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
1741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
1801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
1861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
1921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
1981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
2041 tgacagcggc cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca
2101 ccgatctcag cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag
2161 atcactacga ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag
2221 ccgcgttcac cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga
2281 agaagtggtc ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct
2341 agagtaagaa gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc
2401 gtggtatcac gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc
2461 cgggtcacat gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc
2521 gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat
2581 tctcttaccg tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag
2641 tcgttttgtg agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac
2701 aacaccgcgc cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg
2761 cggaaagact caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca
2821 cccagttgat cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc
2881 aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc
2941 gtcctttttc aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa
3001 gtaagtaata ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc
3061 attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca
3121 aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc
3181 cagaacattt ctct
//
